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SynK / Anti-SynK antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY5111S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

SynK is a thylakoid potassium channel protein, encoded by the SynK gene (slr0498) in Synechocystis sp. PCC 6803. Earlier work shows that SynK is tend to migrate as tetramers even in the presence of SDS.

  Synonyms:

SynK

  Immunogen:

Recombinant protein of SynK (115-205aa) derived from Synechocystis sp. PCC 6803 (slr0498).

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

23.1 / 100 kDa

  Confirmed Reactivity: 

Synechocystis sp. PCC 6803

  Predicted Reactivity: 

For more species homologues information, please contact tech support at arashare01@qq.com.

 

 Application Example

Lane 1: The thylakoid membrane protein of ΔSynK from Synechocystis sp. PCC 6803 cells containing 4 μg chlorophyll.
Lane 2: The thylakoid membrane protein of WT from Synechocystis sp. PCC 6803 cells containing 4 μg of chlorophyll.
Electrophoresis: 12% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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