AraShare科学

Product Information

Description:	Rabbit polyclonal antibody
Background:	ndhA
Synonyms:	ndhA, NAD(P)H dehydrogenase I subunit 1, NDH-1 subunit 1, NDH-A
Immunogen:	KLH-conjugated synthetic peptide of ndhA protein derived from Synechocystis sp. PCC 6803.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Serum Peptide affinity form antibody available upon request at info@phytoab.com.
Reconstitution:	Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Information

Recommended Dilution:	Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected/apparent MW:	40 kDa
Confirmed Reactivity:	Synechocystis sp. PCC 6803
Predicted Reactivity:	For more species homologues information, please contact tech support at tech@phytoab.com.

Application Example

WT(12.5-100) is thylakoid membrane protein from WT of Synechocystis sp. PCC 6803 containing 0.625 μg, 1.25 μg, 2.5 μg, 3.75 μg and 5 μg of chlorophyll, respectively.
A(100-12.5) is thylakoid membrane protein from mutant (ndhA) of Synechocystis sp. PCC 6803 containing 5 μg, 3.75 μg, 2.5 μg, 1.25 μg and 0.625 μg of chlorophyll, respectively.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 2h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.