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PHY0729A ANN1 / Anti-Annexin D1 antibody
价格:¥2540.00 ¥3180.00
积分:
货号:PHY0729A
数量:99
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Annexin (ANN) is a member of a diverse, multigene family of calcium-dependent transporters. There are eight annexins in arabidopsis: ANN1 (AT1G35720), ANN2 (AT5G65020), ANN3 (AT2G38760), ANN4 (AT2G38750), ANN5 (AT1G68090), ANN6 (AT5G10220), ANN7 (AT5G10230), ANN8 (AT5G12380).

  Synonyms:

ANN1, ANNAT1, ANNEXIN 1, ATANN1, ATOXY5, OXY5

  Immunogen:

KLH-conjugated synthetic peptide of ANN1 derived from Arabidopsis thaliana AT1G35720.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Applications: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

36 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Brassica rapa.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

20 μg total protein from Arabidopsis thaliana.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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