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PHT1;1/2/3 / Anti-PHOSPHATE TRANSPORTER 1;1/2/3 antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY0753
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PHOSPHATE TRANSPORTER 1 (PHT1) is an inorganic phosphate transporter. Arabidopsis PHT1 family transporters contain 9 proteins: Pht1;1/AT5G43350, Pht1;2/AT5G43370, Pht1;3/AT5G43360, Pht1;4/AT2G38940, Pht1;5/AT2G32830, Pht1;6/AT5G43340, Pht1;7/AT3G54700, Pht1;8/AT1G20860, Pht1;9/AT1G76430.

  Synonyms:

PHT1;1/2/3

  Immunogen:

KLH-conjugated synthetic peptide of PHT1;1, PHT1:2, PHT1:3 derived from Arabidopsis thaliana AT5G43350/AT5G43370/AT5G43360.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Applications: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

58 kDa (AT5G43350/ AT5G43370), 57 kDa (AT5G43360)

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity:  

For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 20 μg total protein from Arabidopsis thaliana leaf. 
Lane 2: 40 μg total protein from Arabidopsis thaliana leaf. 
Lane 3: 80 μg total protein from Arabidopsis thaliana leaf. 
Electrophoresis: 12% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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