Product Information
Description: | Rabbit polyclonal antibody |
Background: | The vacuolar-type H(+)-ATPase acidifies intracellular compartments and is essential for many processes, including cotransport, guard cell movement, development, and tolerance to environmental stress. There are at least 26 subunits of the vacuolar-type H(+)-ATPase in the Arabidopsis thaliana. The pump consists of subunits A through H of the peripheral V(1) complex, and subunits a, c, c" and d of the V(o) membrane sector. |
Synonyms: | VHA-D, V-ATPase, D |
Immunogen: | KLH-conjugated synthetic peptide derived from C-terminal section of VHA-D protein in Arabidopsis thaliana AT3G58730. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Immunogen affinity purified |
Reconstitution: | Reconstitution with 150µl of 0.01 M sterile PBS. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 29 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Triticum aestivum, Hordeum vulgare, Brassica napus, Brassica rapa, and 80-99% homologues with the sequence in Cucumis sativus, Nicotiana tabacum. |
Application Example
![]() | Lane 1: 20 μg total protein from Arabidopsis thaliana leaf. |