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VHA-D / Anti-Vacuolar H+-ATPase subunit D antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY1330A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

The vacuolar-type H(+)-ATPase acidifies intracellular compartments and is essential for many processes, including cotransport, guard cell movement, development, and tolerance to environmental stress. There are at least 26 subunits of the vacuolar-type H(+)-ATPase in the Arabidopsis thaliana. The pump consists of subunits A through H of the peripheral V(1) complex, and subunits a, c, c" and d of the V(o) membrane sector.

  Synonyms:

VHA-D, V-ATPase, D

  Immunogen:

KLH-conjugated synthetic peptide derived from C-terminal section of VHA-D protein in Arabidopsis thaliana AT3G58730.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

29 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Triticum aestivum, Hordeum vulgare, Brassica napus, Brassica rapa, and 80-99% homologues with the sequence in Cucumis sativus, Nicotiana tabacum.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

Lane 1: 20 μg total protein from Arabidopsis thaliana leaf.
Lane 2: 40 μg total protein from Arabidopsis thaliana leaf.
Lane 3: 40 μg total protein from Arabidopsis thaliana leaf.
Lane 4: 60 μg total protein from Arabidopsis thaliana leaf.

Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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