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FtsH2/6/8 / Anti-Plant fts metallo-protease H2/6/8 antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY1385S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

FtsH6 is an FtsH protease that is localized to the chloroplast. AtFtsH6 is involved in the degradation of both Lhcb3 and Lhcb1 during senescence and high-light acclimation.

  Synonyms:

FtsH2/6/8, ATP-dependent zinc metalloprotease FTSH2/6/8, chloroplastic, AtFTSH2/6/8

  Immunogen:

KLH-conjugated synthetic peptide of FtsH2/6/8 derived from Arabidopsis thaliana AT2G30950, AT5G15250, AT1G06430.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

74 / 66 kDa (AT2G30950), 56 kDa (AT5G15250), 73 / 66 kDa (AT1G06430)

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Spinacia oleracea, Zea mays, Sorghum bicolor.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 1.25 μg, 2.5 μg, and 5 μg of chlorophyll, respectively. 
Electrophoresis: 10% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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